Figure 3.
(A) The natural logarithm of koff,1 (circles) and koff,2 (squares) versus 1/T [Equation (2)] for the two target sequences: PM (red) and MM (blue). (B) The natural logarithm of koff,eff for the same target sequences as in A. No values are reported above 35°C for MM since at these temperatures the hybridization is too weak for sufficient statistics. Multiple temperature sweeps were performed for each DNA target. The error bars represent ± one standard deviation in dissociation rate constant from three independent measurements at each temperature.