Figure 4.
Dicer is an E2-inducible gene. (A) ERα DNA-binding patterns to the genomic region harboring Dicer. Black bars represent ERα-binding sites in two cell types observed under ethanol-treated or E2-treated condition. (B) E2-inducible expression of Dicer in MCF-7p and MCF-7AKT cells was measured by northern blotting (top panel). The same blot was reprobed with 36B4 to ensure integrity of RNA. qRT–PCR confirming E2-inducible expression of Dicer is also shown (mean plus SEM, bottom panel). *P < 0.04 ethanol-treated versus E2-treated cells.
