Table 1.

CopG binding affinity to different operator variants

^aThe four binding sites of the operator (LA, LSE, RSE and RA) are indicated on the schematic representation of the wild-type target DNA (WT). Continuous lines denote wild-type sequence regions. Dashed lines represent DNA regions with substituted sequence. The names of the mutant operators are indicated on the left.

^bThe affinities of binding of CopG to the 55-bp operator variants relative to the 239 wild-type DNA fragment are shown. The number of samples independently analyzed to calculate each relative binding affinity varied between 17 and 33. The calculated relative affinities were normalized for the value of the ratio between the affinity of CopG for the 55-bp wild-type oligonucleotide and the affinity of the protein for the 239-bp wild-type operator DNA fragment (0.85 ± 0.04).