Figure 5.
Extra-loop deletion variants lose the splicing endonuclease activity. (A) GelCode-Blue-stained 15% SDS–PAGE gel. In each lane, 2 μg of the fraction eluted from TALON resin was loaded. (B) Splicing endonuclease assay. Sulfolobus tokodaii tRNATrp precursor was used as the substrate. Lane assignments are; M, molecular mass marker for (A); WT, wild-type 6×His-PAE-EndA; DEL-1, variant DEL-1; DEL-2, variant DEL-2; DEL-3, variant DEL-3. The amount of the protein used for the assay was 0.5 μg.