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. 2009 Jun 11;37(14):4812–4825. doi: 10.1093/nar/gkp509

Figure 5.

Figure 5.

Analysis of expression of selected genes in response to iron and Fur. (A) Expression was determined from cells harboring the respective promoter fusions to a lacZ reporter gene after incubation at 30°C for 2 h in presence of either 100 μM FeSO4 (Fe) or 100 μM 2′-dipyridyl (DDP). The last panel shows as a control the expression of CC2194 in NA1000, the fur mutant (fur) and fur complemented with the gene in trans (fur+), indicating that the presence of Fur restores the wild-type expression. (B) Expression driven by the nuoA and sdhC promoters was determined from cells harboring the respective promoter fusions to a lacZ reporter gene after incubation at 30°C for 2 h in presence of 100 μM FeSO4. The assays were carried out using either the wild-type promoters (P) or the mutagenized promoters (P*) as described in Figure 3, introduced into the NA1000 or SP0057 strains. β-Galactosidase activity is expressed in Miller units (38) and is the average of at least three independent assays.