FIG. 5.

Excitatory inward currents induced by CAR involved the activation of M1, nicotinic cholinergic receptor and probably M3 or M5 receptors in the Pf. A: CAR-induced inward currents were not blocked by mecamylamine (MEC) alone but were blocked during co-pretreatment with pirenzepine (PRZ) and MEC. Note the 2 phases of inward current in the top recording, whereas only 1 phase was present in the 2nd recording. B: inward currents induced by CAR were partially blocked by PRZ or co-application of PRZ and MEC. Note the 2 phases in the 2nd recording. Black bars show the period during which neuroagents were applied, and scale bars are 100 s and 20 pA for the horizontal and vertical axes, respectively.