Skip to main content
. Author manuscript; available in PMC: 2009 Aug 10.
Published in final edited form as: J Immunol. 2007 May 15;178(10):6297–6306. doi: 10.4049/jimmunol.178.10.6297

FIGURE 3.

FIGURE 3

BAC-containing transformants of the IgG2a-secreting cell line 9921. Top, Autoradiograph of DNA from the parental line (9921) and individual transformants (e.g., 6.1, 7.1, etc.) cut with HindIII and hybridized with the probe shown in the diagram below. The estimated number of copies of the BAC in each transformant is shown below the lanes (with the 4.4-kb fragment assigned a value of four copies; see Materials and Methods). Bottom, Diagram of the region upstream of Cα in the endogenous loci of 9921 and in the BACs introduced into this cell line. H (HindIII) recognition sites are shown in the relevant regions.