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. 2009 Jul 28;8:51. doi: 10.1186/1476-4598-8-51

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Copyright © 2009 Abubaker et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Determination of PIK3CA gene copy number by FISH on ovarian tissue (A). B. Sample 1 is normal cell selected according to FISH analysis. Samples 2–4 are PIK3CA amplified ovarian tumor cells selected according to FISH. FISH images show cell nuclei (blue) from selected cases, hybridized with probes directed against PIK3CA gene (green RP11-245 C23) and centromere 3 (red). (1). Normal cell (blue) shows 2 centromeric signals (red) and 2 (green) PIK3CA signals. (2–4). Representative cells show amplification two (red) centromeric signals and green PIK3CA amplified Signals.