Table 1.
Antibodies used for tissue microarray Immunohistochemical analysis
| Antibody (Subcellular Localization) | $Positive cases (%) | Clone | Company | Source | Dilution Overnight in incubation | Retrieval | Detection System |
| PTEN@ | 111/144 77.1% |
6H2-1 | Cascade Bioscience | Mouse monoclonal | 1:100 | pH 9, Pressure Cooker | EnVision+ |
| p-AKT (Cytoplasmic & Nuclear) |
75/144 52.1 |
Ser473 | Cell Signaling | Mouse Mono-clonal |
Predilute | pH 9, microwave | Survival Marker; Signal Stain IHC detection Kit |
| PIK3CA-110 | 78/140 55.7% |
1G12E9 | Everest Biotech | Goat Polyclonal | 1:400 | pH 6, Pressure Cooker | EnVision+ |
| P53 | 68/138 49.3% |
DO-7 | D9K0 | Mouse monoclonal | 1:50 | pH 9, microwave | Ventana Bechmark |
@Normal expression of PTEN was seen in 77.1% cases and PTEN inactivation (loss/reduced expression) was seen in 33/144(22.9%) of EOC cases.
$ Non representative spots for the various antibodies ranged from18 cases for p53 to 12 spots for PTEN IHC