Figure 1.

Study Design – BT474 and Stratagene Universal Human Pooled Reference RNA were used as the substrate for these experiments. 10 ug of total RNA from each were hybridized to microarrays and labeled "total RNA arrays". SAM analysis from these total RNA arrays were used to select QPCR genes in an unbiased fashion prior to performing any amplification reaction. Total RNA was serially diluted, amplified, and hybridized to cDNA microarrays. QPCR was performed on total RNA and amplified RNA. Statistical analyses included microarray vs microarray analysis as well as microarray vs QPCR analysis.