Table 1.

Lower limits of total RNA required for each amplification method.

Amplification technique Modified T7	Amplification yield, BT474 RNA(mcg)
140 pg	0.80 – insufficient
280 pg	0.60 – insufficient
375 pg	0.16 – insufficient
500 pg	1.4 – sufficient for 1 array
500 pg	1.7 – sufficient for 1 array
500 pg	0 – insufficient
500 pg	0 – insufficient
500 pg	1.9 – sufficient for 1 array
1 ng	17.8
1 ng	20.3
Arcturus
100 pg	0 – insufficient
100 pg	0 – insufficient
250 pg	12
250 pg	7.2
500 pg	56.8
1 ng	52.7
Balanced PCR*
500 pg	0 – insufficient
500 pg	1.5 – sufficient for 1 array
667 pg	2.1 – sufficient for 1 array
667 pg	2.0 – sufficient for 1 array
1 ng	0 – insufficient
1 ng	2.0 – sufficient for 1 array
3.3 ng	2.3 – sufficient for 1 array
3.3 ng	3.2

*1 array discarded due to poor hybridization