Table 1.
Lower limits of total RNA required for each amplification method.
| Amplification technique Modified T7 | Amplification yield, BT474 RNA(mcg) |
| 140 pg | 0.80 – insufficient |
| 280 pg | 0.60 – insufficient |
| 375 pg | 0.16 – insufficient |
| 500 pg | 1.4 – sufficient for 1 array |
| 500 pg | 1.7 – sufficient for 1 array |
| 500 pg | 0 – insufficient |
| 500 pg | 0 – insufficient |
| 500 pg | 1.9 – sufficient for 1 array |
| 1 ng | 17.8 |
| 1 ng | 20.3 |
| Arcturus | |
| 100 pg | 0 – insufficient |
| 100 pg | 0 – insufficient |
| 250 pg | 12 |
| 250 pg | 7.2 |
| 500 pg | 56.8 |
| 1 ng | 52.7 |
| Balanced PCR* | |
| 500 pg | 0 – insufficient |
| 500 pg | 1.5 – sufficient for 1 array |
| 667 pg | 2.1 – sufficient for 1 array |
| 667 pg | 2.0 – sufficient for 1 array |
| 1 ng | 0 – insufficient |
| 1 ng | 2.0 – sufficient for 1 array |
| 3.3 ng | 2.3 – sufficient for 1 array |
| 3.3 ng | 3.2 |
*1 array discarded due to poor hybridization