Table 4.

Predicted protein sizes and antibody specificity

Variant	Size (aa)*	Predicted MWt (kDa)	Predicted expression (real-time PCR)
			HASM	HBEC	BEAS2B	PBMC	PMN	THP1
Classical	283	31.5	++	++	+	+++	+++	+
Alternative Exon7	259	28.9	+++	+++	++	+++	-	++
Classical del3	235	26.2	-	-	-	++	++	-
Classical del5+6	189	21.2	-	-	-	++	++	-
Classical del6	234	26.1	++	++	+	+++	+++	+
Alternative del 4+5	160	17.8	+++	+++	++	++	-	+
D1 fragment	83/89	9.8	++	++	++	++	++	++
Classical D2/3	196	21.8	++	++	+	++	++	+
Alternative D2/3	172	19.2	++	++	++	++	-	++

* After removal of signal peptides

Predicted protein translations were performed for variants detected in real-time PCR assays (Figures 4 and 5) and predicted molecular weights (MWt, kDa) obtained. Note that these are potentially about 10 kDa smaller than might be detected at the protein level due to glycosylation. For each variant, relative expression based on real-time PCR results is summarised where +++: highest expression, ++: expression, + low expression and -: not detected. Other variants were detected in RACE experiments, which may result in expression of additional protein variants. Note that the D1 antibody would detect all variants except exon 3 deletions and D2/3 fragments, whilst the D2 antibody would not detect exon 4 deletions or the D1 fragment.