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. Author manuscript; available in PMC: 2010 Aug 25.
Published in final edited form as: Brain Res. 2009 Jun 21;1286:1–12. doi: 10.1016/j.brainres.2009.06.036

Figure 4. Confirmation of siRNA knockdown of FKBP52 and FKBP51 in SH SY5Y cells.

Figure 4

Differentiated SH-SY5Y cells on glass coverslips in 24 well plates were exposed to 250 ng siRNA generated against the FKBP4 (a) gene or against FKBP5 gene (b) and a control gene, encoding GFP provided by the manufacturer, for 24 hr; protein was isolated for Western blot. Concurrent to probing for FKBP52 a western blot for Actin was performed for the loading control (a). To ensure that a difference in GR immunofluorescence was not due to altered GR protein content, GR was assessed by western analysis (c). To discern non-specific bands in the western blots, membranes were developed with primary antibody (d-left), without primary antibody (d-middle) and without either secondary or primary antibody (d-right center), and it was determined that the non-specific band originates from the avidin-biotin amplification system in (a).