Fig. 2.

Antibodies against KDP-1 localize to the NE of germline nuclei. (A) Western blot of worm lysates from wild-type (N2) or transgenic (TG) worms expressing GFP::KDP-1. Blot was probed with pre-immune serum (left) or the affinity-purified KDP-1 antibody (right). Size in kDa is shown on the right. (B) Dissected adult hermaphrodite gonad stained with DAPI to show nuclei. The mitotic zone is the most distal part of the gonad (DG). Stages of meiotic prophase I are marked in orange. Individual bivalents can be seen in diakinesis oocytes at the proximal end of the gonad (PG). DTC is the region covered by the distal tip cell and PCD is the region of the gonad where most programmed cell death occurs. Image is a composite of three gonads; dashed lines correspond to intervening areas not covered by individual gonad images. Adapted from (Lints and Hall, 2005). (C) Single focal plane of fixed wild-type germline nuclei from the mitotic zone through early pachytene probed with anti-KDP-1 (row 1 and red in merge) and anti-nuclear pore complex (row 2 and green in merge) antibodies and stained with DAPI (row 3 and blue in merge). Scale bar: 10 μm. Box represents zoomed region to the right.