Fig. 4.
kdp-1(RNAi) causes germline defects. (A) Western blot of lysates from wild-type (WT; N2) or kdp-1(RNAi) mixed-stage animals probed with anti-KDP-1. Equal loading of the lysates was confirmed by staining with Coomassie blue. (B) Partial projections of DAPI-stained proliferative and transition zones of wild-type (untreated) and kdp-1(RNAi) gonads. Scale bar: 10 μm. (C) Partial projections of DAPI-stained gonads from kdp-1(RNAi), or sun-1(RNAi) animals. Arrows mark abnormal large chromatin masses. Outlines of the gonads have been drawn in grey. Scale bar: 10 μm. (D) Projections of deconvolved images of DAPI-stained diakinesis nuclei of the indicated genotypes. Scale bar: 2 μm. (E) Projection of a DAPI-stained untreated male gonad and a kdp-1(RNAi) male gonad. Scale bar: 10 μm.
