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. 2009 Jan 29;4(3):294–301. doi: 10.1007/s11552-009-9164-0

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Summary flowchart of the steps taken in the study. Summary of the steps taken in the study to determine whether there is differential gene expression levels for genes in DD and control skin, fat, and fascia [39]. Following the consent of all subjects selected for the study, relevant surgical procedures (dermofasciectomy for cases and carpal tunnel release for controls) were carried out, and biopsies of skin, fat and palmar fascia were obtained. The biopsy samples were subjected to RNA extraction, following which cDNA synthesis and quantitative PCR were carried out. Relative gene expression levels were determined using the . ΔC_T was determined by subtracting the threshold cycle of reference gene from those of target genes. Independent t tests were then carried out on relative gene expression levels to determine genes that are significantly (p < 0.05) differentially expressed. ADAM12 A disintegrin and metalloproteinase domain; ALDH1A1 aldehyde dehydrogenase 1 family member; IRX6 iroquois homeoboxprotein 6; PRG4 proteoglycan 4; TNC tenascin C; POSTN periostin, osteoblast specific factor; RPL32 ribosomal protein L32; GAPDH glyceraldehyde-3-phosphate dehydrogenase; ΔC_T delta threshold cycle; PCR polymerase chain reactions; RNA ribonucleic acid; cDNA complementary deoxyribonucleic acid.

Inline graphic — Summary flowchart of the steps taken in the study. Summary of the steps taken in the study to determine whether there is differential gene expression levels for genes in DD and control skin, fat, and fascia [39]. Following the consent of all subjects selected for the study, relevant surgical procedures (dermofasciectomy for cases and carpal tunnel release for controls) were carried out, and biopsies of skin, fat and palmar fascia were obtained. The biopsy samples were subjected to RNA extraction, following which cDNA synthesis and quantitative PCR were carried out. Relative gene expression levels were determined using the . ΔC_T was determined by subtracting the threshold cycle of reference gene from those of target genes. Independent t tests were then carried out on relative gene expression levels to determine genes that are significantly (p < 0.05) differentially expressed. ADAM12 A disintegrin and metalloproteinase domain; ALDH1A1 aldehyde dehydrogenase 1 family member; IRX6 iroquois homeoboxprotein 6; PRG4 proteoglycan 4; TNC tenascin C; POSTN periostin, osteoblast specific factor; RPL32 ribosomal protein L32; GAPDH glyceraldehyde-3-phosphate dehydrogenase; ΔC_T delta threshold cycle; PCR polymerase chain reactions; RNA ribonucleic acid; cDNA complementary deoxyribonucleic acid.