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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mol Immunol. 2009 Jun 11;46(13):2706–2713. doi: 10.1016/j.molimm.2009.05.024

Fig. 2.

Fig. 2

C/EBPα and C/EBPβ bind to the TNFR1 promoter. (A) In vitro translated C/EBPα or C/EBPβ, or reticulocyte lysate control, were incubated with a radiolabeled oligonucleotide probe containing TNFR1 promoter sequences between -94 and -75 in an EMSA. The positions of the bound C/EBPα or C/EBPβ proteins are shown. (B) Nuclear extracts from 293 cells transfected with a C/EBPα expression vector or empty vector control were incubated with the TNFR1 -94/-75 probe or a positive control C/EBP consensus binding sequence. All lanes were run on the same gel, with a vertical black line representing a lane deletion.