Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jun 2;60(12):3337–3352. doi: 10.1093/jxb/erp167

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

PMC Copyright notice

Fig. 3. — Association of LT-B and GFP with starch and fibre fractions of transgenic maize kernels. Transgenic maize kernels expressing constructs presented in Fig. 1 were used for small-scale fractionation. Protein extraction from starch and fibre fractions were used for LT-B and GFP determination. (A) Functional LT-B content as a percentage of TAEP. Error bars correspond to the standard deviation of two technical replicates. (B) Anti-GFP Western blot of starch soluble (TAEP) and insoluble (pellet) phases. (C) Anti-GFP Western blot of fibre soluble (TAEP) and insoluble (pellet) phases. P309, Pγzein-GFP; P310, Pγzein-BSP-LT-B::GFP; P311, Pγzein-ZSP-LT-B::GFP; P315, Pγzein-BSP-GFP; GFP, commercial EGFP standard. Arrowheads, GFP. Open arrowhead, possible truncated form of GFP. Asterisks, LT-B::GFP fusion. Dots, possible cleavage peptides cross-react to GFP antibody. Multiple EGFP bands in GFP standard may be due to incomplete protein denaturation during boiling before loading.