Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jun 16;60(12):3477–3489. doi: 10.1093/jxb/erp183

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

PMC Copyright notice

Fig. 1. — Calcium influx plays an important role in the establishment of cell polarity in monospores. (A) The polarized F-actin and renascent cell wall synthesis in monospores incubated in ESL for 3 h after release from the gametophyte. The polarized F-actin accumulated in the front of the migrating monospores (a) and the renascent cell wall synthesis in migrating monospores (b). The arrow in (a) indicates the direction of migration of the monospore. Left and right photographs in each panel show bright-field and fluorescent images, respectively. Scale bars=5 μm. (B) Effects of calcium chelator (EGTA) and calcium channel blocker (LaCl₃) on the F-actin organization and renascent cell wall synthesis. Freshly released monospores incubated with 1 mM EGTA (a, b) and 100 μM LaCl₃ (c, d) for 3 h, which indicate the symmetrical organization of F-actin (a, c) and no renascent cell wall synthesis (b, d). Upper and lower photographs in each panel show bright-field and fluorescent images, respectively. Scale bars=5 μm. (C) Dose-dependent inhibition of the motility and development of monospores by calcium chelator and channel blocker. Freshly released monospores incubated with an increasing concentration of EGTA (a, c) and LaCl₃ (b, d) for 3 h (a, b) and 24 h (c, d). Columns and vertical bars represent the mean and SD, respectively (n=3).