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. 2009 Jun 16;60(12):3477–3489. doi: 10.1093/jxb/erp183

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© 2009 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 3. — Involvement of PtdIns(4,5)P₂ metabolites in the establishment of cell polarity in monospores. (A) Effects of 2-APB on the motility and development of monospores. Freshly released monospores incubated with an increasing concentration of 2-APB for 3 h (a) and 24 h (b). Columns and vertical bars represent the mean and SD, respectively (n=3). The organization of F-actin and renascent cell wall synthesis in 20 μM 2-APB treated monospores are indicated in (c) and (d), respectively. Upper and lower photographs in each panel show bright-field and fluorescent images, respectively. Scale bars=5 μm. (B) Effects of R59022 on the motility and development of monospores. Freshly released monospores incubated with an increasing concentration of R59022 for 3 h (a) and 24 h (b). Columns and vertical bars represent the mean and SD, respectively (n=3). The organization of F-actin and renascent cell wall synthesis in 15 μM R59022 treated monospores are indicated in (c) and (d), respectively. Upper and lower photographs in each panel show bright-field and fluorescent images, respectively. Scale bars=5 μm.