Figure 7.

The expression of inducible costimulatory molecule (ICOS) in lung and spleen CD4⁺CD25⁺ T cells post adoptive transfer. (A) Contour plots that are representative of the cells in the lung inducible CD4⁺CD25⁻ T-regulatory cells (L25⁻) and spleen inducible CD4⁺CD25⁻ T-regulatory cells (S25⁻) groups. ICOS was expressed at substantially high level in all cockroach-sensitized and -challenged mice with adoptive transfer (CRA^AT+) groups compared with cockroach-sensitized and -challenged mice without adoptive transfer (CRA^AT−) and phosphate-buffered saline (PBS) control. (B) (Left panels) CD4⁺CD25⁺ T cells from lung tissue of all experiment groups. ICOS is significantly upregulated in all CRA^AT+ groups compared with CRA^AT− and PBS control groups with the exception of lung naturally occurring CD4⁺CD25⁻ T-regulatory cells (L25⁺) group. The right panels are from spleen tissue of the same animals that showed high expression of ICOS in L25⁺ and L25⁻ of CRA^AT+ compared with low levels in CRA^AT− and PBS control. *P < 0.01, **P < 0.001.