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. 2009 May 15;180(3):211–225. doi: 10.1164/rccm.200809-1505OC

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Copyright © 2009, American Thoracic Society

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Figure 8. — Expression of TGF-β (400 bp) and IL-10 (610 bp) mRNA transcripts in lung CD4⁺CD25⁺ donor cells and in lung CD4⁺CD25⁺ cells post adoptive transfer. (A) Donor cells: The cells in the lung naturally occurring CD4⁺CD25⁻ T-regulatory cells (L25⁺) and lung inducible CD4⁺CD25⁻ T-regulatory cells (L25⁻) groups expressed low to undetectable levels of IL-10 and TGF-β mRNA transcripts. The cells in the spleen inducible CD4⁺CD25⁻ T-regulatory cells (S25⁻) group expressed low levels of IL-10 and undetectable levels of TGF-β. The cells in the spleen naturally occurring CD4⁺CD25⁻ T-regulatory cells (S25⁺) group expressed low levels of TGF-β and undetectable levels of IL-10. (B) Expression of TGF-β mRNA transcripts in post adoptive CD4⁺CD25⁺ T cells: The cells of cockroach-sensitized and -challenged mice with adoptive transfer (CRA^AT+) in the L25⁺ group exhibited higher expression of TGF-β mRNA transcripts compared with cockroach-sensitized and -challenged mice without adoptive transfer (CRA^AT−) and phosphate-buffered saline (PBS) control. The cells of CRA^AT+ in the L25⁻ group expressed substantially high levels of TGF-β mRNA transcripts compared with undetectable levels in the CRA^AT− group and low levels in PBS control. The cells of CRA^AT+ in the S25⁺ and S25⁻ groups showed high expression of TGF-β mRNA transcripts compared with low levels in the CRA^AT− group and PBS control. (C) IL-10 mRNA transcripts in post adoptive cells: Cells of the CRA^AT+ in the L25⁺ and S25⁺ groups showed a significant increase in IL-10 mRNA transcripts compared with undetectable level in the CRA^AT− and low levels in PBS control groups. Cells of the CRA^AT+ in the L25⁻ group exhibited considerable increase in IL-10 mRNA transcripts compared with undetectable levels in PBS control and moderate levels in CRA^AT− group. Cells of the S25⁻ group the CRA^AT+ exhibited a substantially high level of IL-10 mRNA transcripts compared with low levels in PBS control and CRA^AT− group. Densitometric analyses confirmed the PCR data. *P < 0.01; ** P < 0.001.

Figure 8. — Expression of TGF-β (400 bp) and IL-10 (610 bp) mRNA transcripts in lung CD4⁺CD25⁺ donor cells and in lung CD4⁺CD25⁺ cells post adoptive transfer. (A) Donor cells: The cells in the lung naturally occurring CD4⁺CD25⁻ T-regulatory cells (L25⁺) and lung inducible CD4⁺CD25⁻ T-regulatory cells (L25⁻) groups expressed low to undetectable levels of IL-10 and TGF-β mRNA transcripts. The cells in the spleen inducible CD4⁺CD25⁻ T-regulatory cells (S25⁻) group expressed low levels of IL-10 and undetectable levels of TGF-β. The cells in the spleen naturally occurring CD4⁺CD25⁻ T-regulatory cells (S25⁺) group expressed low levels of TGF-β and undetectable levels of IL-10. (B) Expression of TGF-β mRNA transcripts in post adoptive CD4⁺CD25⁺ T cells: The cells of cockroach-sensitized and -challenged mice with adoptive transfer (CRA^AT+) in the L25⁺ group exhibited higher expression of TGF-β mRNA transcripts compared with cockroach-sensitized and -challenged mice without adoptive transfer (CRA^AT−) and phosphate-buffered saline (PBS) control. The cells of CRA^AT+ in the L25⁻ group expressed substantially high levels of TGF-β mRNA transcripts compared with undetectable levels in the CRA^AT− group and low levels in PBS control. The cells of CRA^AT+ in the S25⁺ and S25⁻ groups showed high expression of TGF-β mRNA transcripts compared with low levels in the CRA^AT− group and PBS control. (C) IL-10 mRNA transcripts in post adoptive cells: Cells of the CRA^AT+ in the L25⁺ and S25⁺ groups showed a significant increase in IL-10 mRNA transcripts compared with undetectable level in the CRA^AT− and low levels in PBS control groups. Cells of the CRA^AT+ in the L25⁻ group exhibited considerable increase in IL-10 mRNA transcripts compared with undetectable levels in PBS control and moderate levels in CRA^AT− group. Cells of the S25⁻ group the CRA^AT+ exhibited a substantially high level of IL-10 mRNA transcripts compared with low levels in PBS control and CRA^AT− group. Densitometric analyses confirmed the PCR data. *P < 0.01; ** P < 0.001.