Fig. 8.

rapGAPB^– mutant cells show defects in prestalk and prespore cell-cell adhesion at the finger stage of development. Structures were disaggregated and the percentage of cells that aggregated after different times counted. (A) Cell-cell adhesion of wild-type (dotted line) and rapGAPB^– cells (solid line). (B) Measurement of overall cell-cell adhesion. rapGAPB^– cells are more adhesive than the wild type. Measurement of prespore and prestalk cell adhesion (psA-RFP or ecmAO-RFP expressing cells, respectively). rapGAPB^– prespore cells are more adhesive than wild-type prespore cells and rapGAPB^– prestalk cells are less adhesive than wild-type prestalk cells. As a consequence rapGAPB^– prestalk and prespore cells show similar adhesion, whereas wild-type prestalk cells are more adhesive than wild-type prespore cells.