Fig. 2.

Emerin-dependent changes in β-catenin activity and localisation in preadipogenic medium conditions. Control and emerin-null fibroblasts were cultured in growth medium or preadipocyte differentiation medium for 72 hours. (A) PCR with primers specific for β-actin and β-catenin. (B) β-catenin activity measured with TOP-FLASH reporters. (C,D,F) Immunoblots of whole-cell extracts with β-actin, total β-catenin and active β-catenin antibodies. (E,G) Cytoplasmic (cyt) and nuclear (nuc) fractions probed for total and active β-catenin. (H) Cells were co-stained with antibodies against active β-catenin and PPARγ. Bars: 10 μm; ^*P<0.05.