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. Author manuscript; available in PMC: 2009 Aug 11.
Published in final edited form as: Biochemistry. 2008 Sep 4;47(39):10394–10406. doi: 10.1021/bi8010658

FIGURE 1.

FIGURE 1

AzBCQ labeling of endogenous PfCRT. (A) AzBCQ labeling of saponin-isolated HB3 parasites with 10 µM AzBCQ for 10 min at pH 5.6; from left, competition with cold CQ at 0, 0.8, 1.6, 2.4, 3.2, and 4 mM (lanes 1–6, respectively). Lanes 1–6 are an avidin blot for detecting AzBCQ, and lanes 7–12 show half of the same samples blotted with anti-PfCRT polyclonal antibody. (B) Avidin (lanes 1–6) and anti-PfCRT (lanes 7–12) immunoblots of HB3 and Dd2 saponin-released parasites reacted with 10 µM AzBCQ at pH 5.2 with a 10 min UV illumination time. The arrow indicates the major immunoreactive band on the anti-PfCRT blot. The asterisk indicates a probable degradative byproduct that retains the AzBCQ binding site: lanes 1–3 and 7–9, HB3; lanes 4–6 and 10–12, Dd2; lanes 1, 4, 7, and 10, no competing CQ; lanes 2, 5, 8, and 11, 2 mM CQ competitor; lanes 3, 6, 9, and 12, 4 mM CQ competitor.