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. Author manuscript; available in PMC: 2009 Aug 11.
Published in final edited form as: Biochemistry. 2008 Sep 4;47(39):10394–10406. doi: 10.1021/bi8010658

FIGURE 7.

FIGURE 7

Competition of AzBCQ labeling of HB3, Dd2, and 7G8 PfCRT by CQ (left), QN (middle), and Art (right). AzBCQ labeling of HB3 (■), Dd2 (▲), and 7G8 (●) PfCRT labeling at varying drug:AzBCQ ratios (x axes in panels B and C). AzBCQ was present in a 50-fold molar excess relative to protein, and the UV illumination time was 10 min. Each data point in B panels C is the average of at least two independent experiments, and two densitometry quantifications were conducted for each set of gels (four in total, standard error of <3% in each case). For avidin blot strips (A; the top strip in each case is HB3, the middle Dd2, and the bottom 7G8): lane 1, no CQ; lanes 2–6, 8-, 16-, 24-, 32-, and 40-fold excesses of CQ relative to AzBCQ, respectively; lane 7, no QN competitor; lanes 8–12, 8-, 16-, 24-, 32-, and 40-fold excesses of QN relative to AzBCQ, respectively; lane 13, no ART competitor; lanes 14–18, 8-, 16-, 24-, 32-, and 40-fold excesses of ART relative to AzBCQ, respectively.