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. 2009 Sep;50(9):1842–1851. doi: 10.1194/jlr.M800588-JLR200

Fig. 1.

Fig. 1.

Characterization of rabbit apolipoprotein CI. A: The 61 amino acid sequence of rabbit apoCI (1_61; bold) and the signal peptide sequence (plain; −1_−26) was deduced from the cDNA sequence (GenBank accession number EU673468). Alignment with the 57 amino acid sequence of mature human apoCI (1_57) and human signal peptide (−1_−26) was conducted by using the Needleman-Wunsch global alignment method (EMBOSS software). B: SDS-PAGE of Mark12 calibration kit (lane 1), purified human apoCI (lane 2), and purified rabbit apoCI (lane 3). Human and rabbit apoCI were purified by chromatofocusing (see Methods). C: The MALDI-TOF/TOF spectrum of the 41TRNWFSETISK51 doubly derivatized rabbit apoCI peptide matches exactly the deduced amino acid sequence in A.