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. 2009 Sep;50(9):1842–1851. doi: 10.1194/jlr.M800588-JLR200

Fig. 2.

Fig. 2.

Comparison of the concentration-dependent effects of human and rabbit apoCI on LPL activity and CETP activity as measured with a lipoprotein-independent (B and C) or a lipoprotein-dependent (D) fluorescent assay. A: Ultracentrifugally isolated human VLDLs were preincubated with increasing concentrations of either purified human apoCI (squares in A) or purified rabbit apoCI (open circles in A), and triglyceride hydrolysis was subsequently induced by the addition of purified bovine milk lipoprotein lipase. Triglyceride hydrolysis activity is expressed as the amount of released nonesterified fatty acids (NEFAs) over a 6 min period. B: Transfer of fluorescent NBD-labeled cholesteryl esters from liposome donors to lipoprotein acceptors was measured in the presence of partially purified human CETP and in the presence of increasing concentrations of either human apoCI (squares in B) or rabbit apoCI (circles in B). C: Transfer of fluorescent NBD-labeled cholesteryl esters from liposome donors to lipoprotein acceptors was measured in the rabbit d 1.21 g/ml infranatant, which was supplemented with increasing concentrations of either human apoCI (squares in C) or rabbit apoCI (circles in C). D: Transfer of fluorescent NBD-labeled cholesteryl esters from liposome donors to endogenous lipoprotein acceptors in total human plasma, which was supplemented with increasing concentrations of either human apoCI (squares in D) or rabbit apoCI (circles in D). Data are mean ± SD of three determinations. *P < 0.05 versus homologous control with no apoCI added (Mann-Whitney).