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. 2009 Sep;50(9):1852–1862. doi: 10.1194/jlr.M800635-JLR200

Fig. 5.

Fig. 5.

PAL upregulates SK. A: C2C12 myotubes were treated with 1.25 mM PAL for 14 h. SK1 and SK2 mRNA expression was determined by quantitative real-time PCR. Data are presented as means (n = 5) ± SEM. For SK1, CTL versus PAL, P < 0.05; for SK2, CTL versus PAL, P > 0.05. B: C2C12 myotubes were treated with 1.25 mM PAL with or without 0.1 μM myriocin for 14 h, and SK1 enzyme activity was determined. Data are mean (n = 2) ± SEM; t-test, two-sample assuming equal variances, CTL versus PAL, P < 0.01; PAL versus PAL-MY, P < 0.05. C: C2C12 myotubes were treated with PAL or oleate at the indicated concentrations for 14 h, and SK1 mRNA expression was determined by quantitative real-time PCR. D: C2C12 myotubes were treated with 1.25 mM PAL at the indicated time course, and SK1 mRNA expression was determined by quantitative real-time PCR. E: C2C12 myotubes were treated with 1.25 mM PAL with or without 0.75 mM oleate for 14 h, and SK1 mRNA expression was determined by quantitative real-time PCR. Data are mean (n = 3) ± SEM. For PAL versus CTL, P < 0.05; PAL-OLE versus PAL, P < 0.01. F: Mice were placed on a high-fat diet or isocaloric low-fat control diet for 16 weeks. Hind limb skeletal muscle was isolated postmortem, and tissue homogenates were used for SK message level. Data are mean (n = 3) ± SEM. CTL, control; OLE, oleate, MY, myriocin; HFD, high-fat diet; LFD, low-fat diet.