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Analysis of Cys211 mutants. A: Microsomes from vector-, hLPCAT1-, or the indicated hLPCAT1 mutant-transfected HeLa cells were subjected to Western blot (5 μg of protein per lane) using anti-FLAG antibody. B: LPCAT and C: LPGAT activities of microsomes from vector-, hLPCAT1-WT-, or hLPCAT1 mutants-transfected HeLa cells were measured. B, C: Values are means ± SD. A–C: Same experiments were performed three times independently with similar results.
