Fig. 4.

Effect of 1-octanol on 27-HC synthesis. Replicate flasks were incubated overnight in medium A. The cells were washed and the flasks incubated for 7 min at 37°C with 4 ml PBS containing 20 mg HPCD to reduce their background 27-HC. One flask was processed immediately (zero-time control), while three other flasks were incubated for 4 h at 37°C with 4 ml medium A plus these additions: bar 1, 0.6% ethanol (final); bars 2 and 3, 0.6% ethanol plus 0.6 mM or 0.8 mM 1-octanol, respectively. The values plotted were corrected for a zero-time value of 2.85 ng 27-HC/mg protein. Data are plotted as means ± average deviation for duplicate determinations. One of two similar experiments is shown.