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. Author manuscript; available in PMC: 2010 Mar 10.
Published in final edited form as: Biochemistry. 2009 Mar 10;48(9):1996–2004. doi: 10.1021/bi801707t

Figure 4.

Figure 4

Isothermal titration calorimetry plots for wildtype, Y410F and E218A MtIPMS. Experiments were performed as described in Materials and Methods. The results from a fit of the data to a One Set of Sites model produced the following values for the binding of L-leucine to the wild-type (N = 0.86 ± 0.02, ΔH = 774 ± 20 cal/mol, ΔS = 27.8 cal/mol/K), Y410F (N = 0.91 ± 0.01, ΔH = 2598 ± 48 cal/mol, `S = 30.1 cal/mol/K) and E218A (N = 0.97 ± 0.01, ΔH = 1486 ± 31 cal/mol, ΔS = 28.5 cal/mol/K) enzymes.