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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Cancer Res. 2009 May 12;69(10):4192–4201. doi: 10.1158/0008-5472.CAN-09-0042

Fig. 1. PTEN loss increases PIP3 and P-AKT, and alters ER and PR levels.

Fig. 1

A) Cells were treated with medium containing DCC-FBS (MCF-7: 2%; T47D, MDA-361: 0.5%) × 24 hrs, and lysates were analyzed by immunoblotting with the indicated antibodies. PR was not detected in MDA-361 cells. B) MCF-7 lines were metabolically labeled with 32P-orthophosphatase in 10% dialyzed FBS × 16 hrs. Lipids were extracted, resolved by thin-layer chromatography, and 32P-PIP species were detected by autoradiography (arrows). Origin of spotting is indicated. Cell lysates were also used for immunoblotting to confirm PTEN status. Fold-changes in PR isoforms (A) and PIP3 (B) normalized to actin were determined by densitometry analysis (bar graphs).