Figure 2.

Targeted treatment with morpholino oligonucleotides downregulates PTBP1 and PTBP2. (A) Diagram illustrating the mechanism whereby targeting the exon 2 splice site induces skipping in either PTBP1 or PTBP2 transcripts through blocking of U1 snRNP binding to create mRNAs containing premature stop codons. (B) RT–PCR results for PTBP1, PTBP2 and GAPDH (loading control) after indicated morpholino treatments. Knockdown treatments included: control oligo (C), PTBP1-specific oligo (P1), PTBP2-specific oligo (P2), and a combination of both PTBP1- and PTBP2-specific oligos (P1,2). The asterisk denotes a heterodimer band between exon 2 +/− PCR products. (C) Western blot of PTBP1, PTBP2 and the actin loading control levels after the morpholino treatments described above. The observed PTBP1 doublet is derived from alternative mRNA isoforms.