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. 2008 Dec 2;132(3):778–787. doi: 10.1093/brain/awn330

Figure 2.

Figure 2

Localization of MOR mRNA in the RVM. (A) A representative photomicrograph of neurons containing MOR mRNA in the RVM region using a digoxigenin-labeled cRNA probe of the rat MOR and detected by an anti-digoxigenin antibody and NBT/BCIP (dark blue), counterstained with Mayer's hematoxylin (blue nuclei). Scale bar = 50 μm. (B) Coronal maps of neurons containing MOR mRNA. Tracings are arranged at successive caudal (1) to rostral (8) intervals (120 μm) as for Fig. 1C. Each dot denotes one labeled neuron. Red line delineates the approximate location of the RVM including GiA and raphe magnus. Thin black lines delineate the approximate location of facial nuclei (VII), pyramids (py) and LPGi lying between VII and RVM. Gi, nucleus reticularis gigantocellularis.