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. Author manuscript; available in PMC: 2009 Aug 11.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2009 Feb 26;29(5):732–738. doi: 10.1161/ATVBAHA.108.183210

Figure 2. DFO inhibits the LPS or iron-induced increase in cellular iron and heme levels.

Figure 2

Figure 2

HAEC were incubated for 24 hrs without (control) or with 5 μg/mL lipopolysaccharide (LPS), or treated for 24 hrs with 100 μmol/L DFO and then incubated for 24 hrs with LPS (DFO+LPS). In addition, HAEC were incubated with 100 μmol/L ferric citrate for 48 hrs (Fe) or for 3 hrs with 100 μmol/L ferric citrate followed by addition of 100 μmol/L DFO and incubation for another 45 hrs (DFO+Fe). After incubation, the cells were lysed and assayed for total iron (panel a) or heme (panel b) as described in Methods. Panel a: *P<0.05 vs. control; #P<0.05 vs. Fe or LPS; n=4. Panel b: *P<0.05 vs. control; **P<0.01 vs. LPS; n=4.