Figure 5. LPS time-dependently increases gene expression of p22phox, which is inhibited by DFO, but has no effect on p47phox and NOX4 gene expression.
HAEC were incubated for up to 24 hrs with 5 μg/mL LPS, without or with 24-hr pretreatment with 100 μmol/L DFO. After incubations, the cells were lysed and total RNA was extracted and subjected to real-time RT-qPCR for NOX4 (panel a), p47phox (panel b) or p22phox (panel c) as described in Methods. Results are expressed as fold change vs. control (0 hr LPS). Panel c: *P<0.05 vs. 0 hr LPS; #P<0.05 vs. 3 hrs LPS; n=3, each determined in duplicate.