Table 1.
MLPA probes used in this study to screen isoniazid-resistant mutants
| MLPA probe | Target | Length of ligated probes | Phenotype |
|---|---|---|---|
| 16S rRNA | 16S rRNA gene | 202 bp | member of MTB complex |
| katG-315 | katG codon 315 ACC | 160 bp | isoniazid-resistant via katG-S315T |
| inhA-15 | inhA (-)15 ‘T’ allele | 178 bp | isoniazid-resistant via inhA-C(-)15T |
| katG-463 | katG codon 463 CGG | 319 bp | PGG 2 or 3 |
Indicated are the name of the probe, the region or point mutation it targets, the length of the ligated probes and the phenotype that is associated with the point mutation.