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Sub1 interacts with components of the Pol III transcription machinery. rSub1 or rPC4 (A), subunits of rTFIIIB (B) or of rTFIIIC (C), or rTFIIIA (D) were subjected to SDS/PAGE, transferred onto a membrane, stained with Ponceau S (lanes P) and then probed with 35S-labeled Sub1 (lanes 35S). Labeled protein complexes were revealed by autoradiography.
