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. Author manuscript; available in PMC: 2009 Aug 11.

Published in final edited form as: Cancer Res. 2008 Jun 1;68(11):4331–4339. doi: 10.1158/0008-5472.CAN-08-0943

30d TCM exposed hMSCs migrate towards and support the growth of tumor cells and express markers for myofibroblast lineage. A, Figure shows increased number of 30d TCM exposed hMSCs migrating toward the MDAMB231 cells placed at the bottom of the transwell chamber as compared with naïve MSCs or the 5-aza treated cells. Interestingly, hMSCs exposed to TCM for periods up to 25 days did not show an increase in number of migrating cells as compared to the number of migrating naïve hMSCs. Migration of 30d TCM exposed cells were significantly increased as compared naïve hMSCs (p=0.003) as well as to all other cell types (p<0.005). B, Increase in luciferase activity reflects growth of MBA-luc cells in a mixed culture assay. Luciferase activity was measured to determine growth of MDA-luc cells in the coculture assay. 5-aza treated hMSCs promote growth of MDAMB231 human breast cancer cells in vitro as compared to naïve hMSCs. 1 and 5d TCM-exposed hMSCs do not increase growth of MDAMB231 cells. A significant increase is observed with d10-d20 TCM hMSCs and an additional increase is seen with d25 & d30 TCM exposed hMSCs. C, The quantitative expression of myofibroblast marker proteins α-SMA, FSP and vimentin in naïve hMSCs and 30d breast cancer TCM activated hMSCs were analyzed by immunofluorescence staining (see methods for details).

30d TCM exposed hMSCs migrate towards and support the growth of tumor cells and express markers for myofibroblast lineage. A, Figure shows increased number of 30d TCM exposed hMSCs migrating toward the MDAMB231 cells placed at the bottom of the transwell chamber as compared with naïve MSCs or the 5-aza treated cells. Interestingly, hMSCs exposed to TCM for periods up to 25 days did not show an increase in number of migrating cells as compared to the number of migrating naïve hMSCs. Migration of 30d TCM exposed cells were significantly increased as compared naïve hMSCs (p=0.003) as well as to all other cell types (p<0.005). B, Increase in luciferase activity reflects growth of MBA-luc cells in a mixed culture assay. Luciferase activity was measured to determine growth of MDA-luc cells in the coculture assay. 5-aza treated hMSCs promote growth of MDAMB231 human breast cancer cells in vitro as compared to naïve hMSCs. 1 and 5d TCM-exposed hMSCs do not increase growth of MDAMB231 cells. A significant increase is observed with d10-d20 TCM hMSCs and an additional increase is seen with d25 & d30 TCM exposed hMSCs. C, The quantitative expression of myofibroblast marker proteins α-SMA, FSP and vimentin in naïve hMSCs and 30d breast cancer TCM activated hMSCs were analyzed by immunofluorescence staining (see methods for details).