Table 2.

The targeted genes and PCR primers used for the detection and the differentiation of Cp. abortus, Cp pecorum and C. burnetii.

Target gene	Primers name	Primers sequence (5'-3')	Amplified fragment length (bp)	Melting temperature (°C)
pmp 90/91	pmp-F	CTCACCATTGTCTCAGGTGGA	821	64
	pmp-R821	ACCGTAATGGGTAGGAGGGGT		66.3
CPC	Cpc-F	TTCGACTTCGCTTCTTACGC	526	64.3
	Cpc-R	TGAAGACCGAGCAAACCACC		67.4
IS1111a	Trans-1	TATGTATCCACCGTAGCCAGT	687	67.5
	Trans-2	CCCAACAACACCTCCTTATTC		66

The name, the sequence, the target gene and the predicted amplified fragment, as well as the melting temperature are listed.