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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Med Res Rev. 2009 Sep;29(5):683–741. doi: 10.1002/med.20151

Table VII.

Yields of MNIC adducts (in pmol) in the cellular fractions of a single 75 cm2 flask of cultured confluent endothelial cells. Trapping proceeded for 20 min at 37° C. The second row gives the preincubation times τinc of the supplements. During preincubation the cells were kept at 37° C in an atmosphere with 5% CO2 and 20% O2. (adapted from(19), with permission)

Basal unstimulated CaI (5 μM) NLA (57 μM) L-NAME (50 μM) Imidazole (10 mM) Oxypurinol (100 μM) NaNO2 (250 μM)
τinc -- -- 20′ 2′ 1′ 20′ 20′
In presence of oxygen 110±8 400±30 51± 8 85± 10 n.d. 116±8
20 min of anoxia 160± 10 n.d. 61±8 80±10 170± 10 154±8

below detection limit of ca 10 pmole.