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. 2009 Aug 21;4(8):e6719. doi: 10.1371/journal.pone.0006719

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Yassin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic drawings of the KBTBD10 and PLN promoters showing transcriptionally active regions identified in Fig. 4 above. The heavy lines indicate the segments amplified in the ChIP assay. (B) Chromatin was immunoprecipitated with anti-Flag antibody from K562 cells nucleofected with either pTracer/CMV-Bsd vector (control), or vector containing Flag-tagged NUP98-HOXA9 (NH9) or NUP98-HOXA9/N51S (NH9/N51S). Segments of the KBTBD10 and PLN promoters (indicated in 5A above) were amplified by PCR. The upper lanes show immunoprecipitated chromatin (IP) and the lower lanes show input chromatin (Input).