Table 1. Dissection of the effects of NUP98-HOXA9 on hematopoietic differentiation based on cell morphology.

	Control	NUP98-HOXA9	NUP98-HOXA9/N51S	HOXA9	HOXA9ΔN
Primitive cells %	1.2±0.9	21.6±6.4**	5.2±3.0	7.0±3.7*	4.7±1.8
Intermediate erythroid %	2.8±0.8	16.9±2.1**	12.1±6.0	3.9±3.2	1.5±0.2
Mature erythroid %	23.7±7.9	33.8±10.2*	45.6±11.5*	14.3±12.5	6.5±2.4
Intermediate myeloid %	16.8±0.7	14.0±3.3	11.1±4.0*	33.6±7.2*	18.1±3.4
Mature myeloid %	55.6±6.2	13.7±3.9**	26.1±4.7**	41.3±6.4*	66.4±6.2
Total cells×106	7.2±1.3	11.1±0.7**	9.7±2.4*	5.0±3.1	3.4±0.6

^* P<0.05.

^** P<0.01.

Averages from 3–4 independent experiments are shown; cells from 2 CFC plates for each experimental condition were harvested. The bottom line shows the average total cell numbers in the two plates±standard deviations. Cytospins smears were prepared and stained with Giemsa; a 500 cell differential count was performed. Cells with blast and promyelocyte morphology were counted as primitive; those with myelocyte/metamyelocyte morphology as intermediate myeloid; those with band, segmented neutrophil, monocyte, and macrophage morphology as mature myeloid; those with intermediate hemoglobinization as intermediate erythroid; and those with full hemoglobinization as mature erythroid. The first 5 rows show average percentages±standard deviations. The P value was obtained by comparing to control using a paired two-tailed distribution t-test.