Figure 4. Positional diversities and amino acid preferences of EETI loop 2-substituted clones.

Positional diversities of the (A) EL2-7 and (B) EL2-9 libraries before sorting (grey) and after enriching for trypsin-binding clones (black) are depicted in the upper panels. A diversity score of 0.05 denotes complete conservation while a score of 1.0 signifies the presence of all 20 amino acids in equal proportions. Preferred amino acids at each loop-substituted position of enriched libraries are shown in the lower panels, with amino acids colored according to chemical property: polar (green), basic (blue), acidic (red), external polar (purple), and hydrophobic (black).