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. 2009 Aug 21;4(8):e6724. doi: 10.1371/journal.pone.0006724

Figure 1. Generation and characterization of NPCs from neonatal mice.

Figure 1

(A) Derivation of mouse NPCs. (a) Primary culture of NPCs of neonatal mice carrying the EGFP transgene. (b) Neural spheres were formed in suspension culture. (c) NPCs grew out of neural spheres in adhesive culture. (d)–(f) Corresponding EGFP images of (a)–(c). Scale bars are 50 µm in (a) and (d), 100 µm in (b), (c), (e) and (f). (B) Immunofluorescence staining with antibodies against Sox2, Nestin, Olig2 and Tuj1. Green color indicates EGFP-transgene. Scale bars are 25 µm. (C) Quantitative data of Sox2, Nestin, Olig2 and Tuj1 positive cells in primary NPCs were shown from three independent experiments. (D) RT-PCR results of early NPC markers were shown. Mouse ES (mES) cells were used as a negative control.