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. 2009 Aug 21;4(8):e6724. doi: 10.1371/journal.pone.0006724

Figure 2. Derivation of HFF cells and the flow diagram for iPS induction.

Figure 2

(A) Derivation of HFF cells. (a) HFF cells displayed homogeneous morphology when they grew and migrated out from foreskin tissue clumps. (b) HFF cells at early and (c) late passages. (d–f) Immunofluorescence staining of HFF cells with the antibody against VIMENTIN. Scale bars are 100 µm. (B) RT-PCR results of HFF cells using human LIF and IL6 specific primers. (C) Flow diagram of iPS induction in this study. Tg, transgene.