Figure 4. Spontaneous differentiation ability of iPS cells.

(A) Well-shaped EBs could be formed in suspension culture (a) and maintain expression of EGFP (b). Scale bars are 100 µm. (B) During EB formation processes, various pluripotency markers and differentiation markers were determined using RT-PCR. (C) When iPS cells were cultured in the absence of the HFF cells, various differentiated cells could be observed, such as definitive endoderm-like cells (a) and (b), fibroblast-like cells (c), cardiomyocytes (d), epidermis-like cells (e) and neural rosettes (f). Scale bars are 100 µm in (a), (b), (c) and (e), 50 µm in (d) and (f).