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. 2009 Aug 20;4(8):e6688. doi: 10.1371/journal.pone.0006688

Figure 10. DIDS Inhibits Curcumin-Induced Fibroblast Apoptosis and AIF Translocation to the Nucleus.

Figure 10

Cells were seeded on diagnostic microscope slides and treated with 25 µM curcumin, 500 µM DIDS, the combination, or were left untreated. A–D) Cell morphology after 24 hours of treatment. E–H) Immunocytochemistry of AIF. Cells were fixed and permeabilized and subsequently stained using a specific Ab against AIF. Detection was done via an Alexa-488 labeled secondary Ab. A+E) untreated, B+F) 500 µM DIDS, C+G) 25 µM curcumin, D+H) 25 µM curcumin+500 µM DIDS. I) Flow cytometry data. Cells were collected and stained with Annexin-V-FITC and propidium iodide and quadrant analysis after flow cytometry was performed. Shown are the mean±SD from the living, early and late apoptotic cell fractions from 3 independent experiments, *** = p<0.001 and ** = p<0.01 compared to 25 µM curcumin.